Knowhow offering
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| Title | Insulin Detemir |
| Knowhow is available for | Production of Insulin precursor in the yeast Pichia pastoris . |
| Summary | 1. Preparation of desb30: DesB30 by protease digestion of recombinant Human Insulin Precursor is performed at 2.3 grams scale with the yield of at least 50%
2. Preparation of linker: Preparation of Tetradecanoic acid 2,5-dioxo-pyrrolidin-1-yl active ester linker (Myr-OSu) is performed at 10 grams scale with the yield of at least 60%
3. Synthesis and detemir purification: Attachment of Myr-OSu linker to DesB30 Human Insulin (1.5 gram scale) is performed to obtain Insulin Detemir. Preparative RPHPLC separation/purification of Detemir yields at least 20% in respect to recombinant Human Insulin Precursor.
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| Advantages | 1. Three technologies developed from the insulin precursor: short lasting insulin and two long-lasting insulins (detemir and degludec).
2. Achieved yield of 30-40 g (Insulin precursor) in 10L bioreactor.
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Knowhow is listed under following categories
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Knowhow from
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| Scientific/ engineering subject areas |
Life sciences/ biosciences & engineering |
| Investor interest categories |
Biotechnology/ Biomedical/ Health Technologies |
| Industries |
Pharmaceuticals, Bulk Drugs, Formulations |
| Customer categories and nature of business |
Businesses and other industries (B2B) |
| Technology readiness levels
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Related documents:
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(Local uploaded copy)
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Database reference
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| Database record number |
20220309071047 |
| Date of upload |
10 / Mar / 2022 |
| Date of update |
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| URL to site when communicating about this knowhow |
http://techex.in/khdb/viewrecord.php?recordno=20220309071047 |
| Request for this technology |
Click here for Request Form |
